In order to make clear the tree species of wood used for the Manjusri Bodhisattva statue in the collection of Dali Museum, wood anatomy and DNA barcoding technologies were used for comparison and identification. The result of anatomical comparison shows that the microstructural characteristics of the wood used for the Manjusri Bodhisattva statue were similar to those of the Cinnamomum glanduliferum specimen, and the main cell morphological characteristics were not significantly different from those of Cinnamomum glanduliferum, so it could be preliminarily determined that the tree species of wood used for the statue belonged to the genus, Lauraceae Cinnamomum, but it could not be identified as Cinnamomum glanduliferum. Therefore, further identification was carried out using DNA barcoding. The result of DNA barcode comparison shows that among the five DNA barcodes used for the comparison between the Manjusri Bodhisattva statue and the Cinnamomum glanduliferum specimen, rpoC1, rbcL and matK had low resolutions and could only identify the samples to the genus level, but trnH-GUG and psbA-trnH had high resolutions and could identify the samples as being Cinnamomum glanduliferum. The result of neighbor-joining (NJ) phylogenetic analysis shows that the two wood samples and the selected Lauraceae tree species could be accurately clustered. The DNA barcoding technology could achieve rapid and accurate identification of the tree species of wood used for the Manjusri Bodhisattva statue in the collection of Dali Museum.