In our study, the chemical compositions and microscopic morphology of three glass beads of the late Warring States Period excavated from the Xinzhou Cemetery, Hunan Province, were analyzed using X-ray fluorescence spectrometry and 3D optical microscopy. The results show that these glass-bead samples could be divided into two types according to their chemical compositions, K₂O-CaO-SiO₂ and Na₂O-CaO-SiO₂, both of which were colored with Cu. Through a comparative study of glass beads excavated from other regions of the same period, it was believed that the M1:12 and M1:17 glass beads were made locally in China, and that the M1:18 glass bead was imported.