Inlaid objects of the Qing Dynasty are numerous and varied, and glass is one of the important materials for inlays. In this study, we analyzed the composition and manufacturing techniques of six glass inlays on a Qing Dynasty jade-inlaid hanging screen with a red sandalwood frame from the Palace Museum. Micro-area X-ray fluorescence spectrometry, scanning electron microscopy, micro-Raman spectrometry and laser-induced breakdown spectrometry were applied to the analyses of composition and microstructure. The results show that the samples belong to the K₂O-CaO-SiO₂ system. SiO₂ is introduced with quartz as the glass matrix, K₂O is introduced with niter as the flux and CaO has two main sources: calcite and fluorite. Dark blue glass is colored with cobalt, light blue glass is colored with copper and blue-green glass has copper and iron as its coloring agents. The opacifier for the four glass inlays is CaF₂. Lead-tin yellow is both the coloring agent and opacifier of yellow glass inlays. According to microscopic appearances and archives, it is considered that these glass inlays were made through secondary processing which adopted the traditional jade-manufacturing technique on the glass block.