In order to determine whether the black porous lump on the wall at the tomb of Wuhuifei was wall painting binding media, we used several analytical methods, including optical microscopy, scanning electron microscopy with energy dispersive Xray (SEMEDX), microRaman spectroscopy, FTIR spectroscopy and gas chromatography (GCFID)to analyze this material. The morphology and material composition of the substrate was determined. We found that the lump has carbonized, but there still remains some fresh binding medium. The binding media turned out to be animal glue. Trace of a red pigment made from cinnabar was also found. The reliability of these results is discussed in comparison with previous research documents and ancient books. This study may enrich the archaeological research on mural painting materials of the Tang Dynasty and provide a scientific basis for future restoration.