Experiments were done to exam the inhibitory effect of enzymeson erosive filamentous fungi in the soil, word walls and canoes at the Crosslake Bridge ruins in Xiaoshan. Classical methods were used to separate and identify the fungi, and the suitable concentrations of antifungal reagents were investigated. Aspergillus niger, Aspergillus terreus, Penicilliumpatulum and Penicillium cyclopium were found in the air. Aspergillus niger, Aspergillus terreus, Aspergillus sydowii, Aspergillus versicolor, Penicillium cyclopium, Penicillium patulum, Penicilliumnigricans, Paecilomycesvarioti and Cladosporium were found in the soil. Aspergillus versicolor was found on the wordwall, and Cladosporium on the canoe. The different filamentous fungi have different reactions and tolerances to the enzyme reagents. Chitinasecan inhibit only the growth and development of filamentous fungi, while the composite synergies of chitinase and glucanase resulted in more effective inhibition.