Contamination by mold is a major potential risk for ancient Chinese calligraphy and paintings on display in museums. The isolation and identification of these microorganisms is helpful for developing a useful approach to control deterioration. In this study, the traditional pure culturedependent method was performed, and 8 strains of mold were isolated from the paper paintings. In addition to typical mold characteristics, they were identified by ITS sequencing. The results of the morphological and molecular biological investigations show that Aspergillus spp.,Rhizopus spp. and Trichoderma spp. are present in these in these specimens.