In our study, energy dispersive X-ray fluorescence (EDXRF) spectrometry, confocal laser Raman microspectrometry (LRS), optical coherence tomography (OCT) and optical microscopy (OM) were combined to analyze seven glass eye beads unearthed in ancient Qufu City of the State of Lu, Shandong Province. The chemical compositions, mineral phases, surface physical structures and microstructures, glass types, manufacturing techniques, provenances and functions of the glass eye beads were determined. The results obtained show that they were made in the state of Chu and then transported to the State of Lu as luxury goods. These eye beads are physical evidence of exchanges and contacts between the States of Lu and Chu.