The gilded lacquer gauze is a unique interior architectural decoration found on the first floor of Fu Wang Ge which is located in the Qianlong Garden of the Forbidden City. In order to study its micro-stratigraphy and the organic/inorganic materials present in each paint layer, we applied a variety of analytical methods including cross-sectional microscopy, scanning electron microscopy-energy dispersive spectrometry (SEM-EDS), micro-Fourier transform infrared (mFTIR) spectrometry, pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), gas chromatography/mass spectrometry (GC/MS), and high performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS). A combined interpretation of the data allowed us to re-establish the techniques of lacquer gauze, and also provided sound scientific directions for its conservation.